ISOLATION, PURIFICATION AND PARTIAL CHARACTERIZATION OF UROKINASE FROM COW URINE OF INDIAN ORIGIN (BOS INDICUS)

Cow, Bos indicus is a most valuable animal in all veda and it is called as the Mother of all

Pankaj Dangre; Sathish Shirolkar; Ravindra Badhe; Mayur Bhujbal

2014

Scholarcy highlights

  • Cow, Bos indicus is a most valuable animal in all veda and it is called as the Mother of all
  • The purified pooled urokinase fraction was analyzed by SDSPAGE, the result observed consisted of a single polypeptide chain of high molecular weight urokinase with an apparent molecular weight of 59 kD band in Lane A.
  • Lane A contains the 59 kD band of single chain high molecular weight urokinase chain taken from the pooled fraction
  • Lane B contains the medium molecular marker used as the standard on a 12.5 % SDS-PAGE
  • The precipitated protein fraction of cow urine was dialyzed in dialysis membrane for 24 h, after dialysis sample were tested for total protein content
  • We have made an attempt to investigate the presence of proteins in cow urine
  • The SDS-PAGE analysis of dialyzed cow urine sample shows band identical to the standard

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