Green synthesis of fluorescent carbon dots from carrot juice for in vitro cellular imaging

We report the use of carrot, a new and inexpensive biomaterial source, for preparing high quality carbon dots instead of semi-conductive quantum dots for bioimaging application

Yang Liu; Yanan Liu; Mira Park; Soo-Jin Park; Yifan Zhang; Md Rashedunnabi Akanda; Byung-Yong Park; Hak Yong Kim


Scholarcy highlights

  • Fluorescent carbon nanodots, an intriguing carbon nanomaterial, have been extensively applied in optoelectronic devices, photocatalysis, electrocatalysis, biosensing, and bioimaging owing to their unique structural features and excellent optical and biochemical characteristics
  • Carrot juice was employed as a natural carbon source to be used in the hydrothermal method, which is a generally acceptable approach that is frequently used in calcination of natural sources
  • Carrot juice was employed as a novel natural source in conjunction with the commonly used hydrothermal method to derive carbon dots possessing down and up-conversion PL features
  • The physiochemical and optical properties of CDs were investigated by electron microscopy, fluorescent spectrometers, confocal laser scanning microscopy, and other spectroscopic methods
  • The cell viability of HaCaT cells was higher than 85% even at higher concentration after 24 h incubation against the purified CD aqueous solution
  • The excellent optical properties, biocompatibility, and low cytotoxicity as well as good cellular imaging capability of the carrot juice-derived CDs are highly desired in biomedical applications

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