Isolation of Andrographolide from Andrographis lineata Wall. ex Nees var. lawii C.B. Clarke and its Anticancer Activity against Human Ovarian Teratocarcinoma

Andrographolide is a well-known anticancer phytochemical often isolated from Andrographis paniculata Nees.

Medha A Bhat; Hosakatte Niranjana Murthy

2021

Scholarcy highlights

  • Andrographolide is a well-known anticancer phytochemical often isolated from Andrographis paniculata Nees.
  • This study was targeted to isolate the andrographolide from the leaves of ALw and to assess its role inthe induction of apoptosis against the human ovarian teratocarcinoma cell line
  • Column chromatography, thin-layer chromatography, preparative TLC were used for the isolation and purification while melting point, ultraviolet-visible spectroscopy, Fourier transform infrared, proton nuclear magnetic resonance, carbon-13 nuclear magnetic resonance analysis were carried out for characterization of the compound. 3-(4,5-dimethylthiaxo-2yl) 2, 5-diphenyl tetrazolium bromide assay was carried out for cytotoxicity test and further Annexin-V staining, caspase 3 activity, B-cell lymphoma-2 activity, cell cycle analysis, and DNA damage study by terminal deoxynucleotidyl transferase nick end labeling assays were carried out for apoptosis study
  • The above data were comparable to standard andrographolide as well as earlier reported results18. 1H NMR was found to be δ ppm 6.59, 5.70, 5.05, 4.82, 4.59, 4.34, 4.04, 3.79, 3.20, 2.26, 1.79, 1.31, 1.18, 1.04, 0.62 and a characteristic gamma lactone chiral carbon proton attached to –OH group had resonated at 6.579 ppm as triplet of doublet. 13C-NMR:δ ppm 170.49, 148.12, 146.84, 129.50, 108.80, 78.95, 74.87, 74.79, 65.03, 63.16, 56.00, 54.88, 42.80, 39.11, 38.03, 37.02, 28.41, 24.48, 23.61, 15.27. 13C-NMR data presented of andrographolide isolated from ALw were correlating with earlier reports
  • The results obtained at the end of the study conveyed that Andrographis lineata Wall. ex Nees var. lawii C.B
  • The andrographolide isolated was a potent anticancer agent against ovarian teratocarcinoma leading to further studies in this regard

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