Oral and Rectal Administration of Bacteriophages for Control of Escherichia coli O157:H7 in Feedlot Cattle

This study compared oral and rectal administration of O157-specific bacteriophages for mitigating the fecal shedding of Escherichia coli O157 by experimentally inoculated steers

ERICA A. ROZEMA; TYLER P. STEPHENS; SUSAN J. BACH; ERASMUS K. OKINE; ROGER P. JOHNSON; KIM STANFORD; TIM A. McALLISTER

2016

Scholarcy highlights

  • This study compared oral and rectal administration of O157-specific bacteriophages for mitigating the fecal shedding of Escherichia coli O157 by experimentally inoculated steers
  • Fecal shedding of nalidixic acid–resistant E. coli O157:H7 was monitored over 83 days after oral, rectal, both oral and rectal, or no treatment with a four-strain O157-specific bacteriophage cocktail in multiple doses
  • Bacteriophages were enumerated by plaque assay, and NalR E. coli O157:H7 by direct plating on sorbitol MacConkey agar supplemented with cefixime, potassium tellurite, and nalidixic acid
  • Bacteriophage was isolated from CON steers, indicating that these steers acquired the bacteriophage from the environment and shed the phage at a level similar to that of REC steers
  • Continuous bacteriophage therapy may be an efficacious method for mitigating shedding of E. coli O157:H7 in cattle, providing that the host bacterium does not develop resistance
  • This therapy may be especially advantageous if nontreated cattle can acquire this biocontrol agent from the feedlot environment

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