In vitro cloning of Bambusa pallida Munro through axillary shoot proliferation and evaluation of genetic fidelity by random amplified polymorphic DNA markers

Arunachal Pradesh Forest Research Institue, on in vitro cloning of B. pallida through axillary Itanagar, North Eastern India, based on the shoot proliferation from the explants of superimorphological traits as well as by the Rain or genotypes of mature clumps and evaluation Bambusa pallida Munro is one of the most Forest Research Institute, Jorhat. In vitro of genetic fidelity of micropropagated plants industrially important bamboo species

Beena D.B.; Rathore T.S.

2012

Scholarcy highlights

  • Arunachal Pradesh Forest Research Institue, on in vitro cloning of B. pallida through axillary Itanagar, North Eastern India, based on the shoot proliferation from the explants of superimorphological traits as well as by the Rain or genotypes of mature clumps and evaluationBambusa pallida Munro is one of the most Forest Research Institute, Jorhat. In vitro of genetic fidelity of micropropagated plants industrially important bamboo species
  • Fortified with additives and various concentra- Genetic fidelity was carried out in 25 mL of reaction mixture tions of auxins and cytokinins [benzy- axillary shoot proliferation from the 1-year old, 220 μM each of dATP, laminopurine, 0.3 μM
  • Among the various concentrations of auxins and cytokinins tested, combination of naphthalene acetic acid and BAP/TDZ in the medium had a significant effect on shoot multiplication
  • Propagation of bamboo through offset cuttings, rhizome splite ting, air layering, culm cuttings and culm s branch cuttings are the traditional methods of u vegetative propagation
  • These methods are l limited by the short period of availability of cuttings at the appropriate stage, the low rate ia of rooting from culm and branch, the need for c bulk quantities of material and the potenr tially small-scale production
  • Survival rate during hardening was more than 95%
  • Microprom pagation of bamboo species from the explants of field grown clumps have been reported in a m few species but there have been no reports on o the evaluation of genetic fidelity of micro-propc agated bamboo plants by molecular markers. - Murashige and Skoog liquid medium supplemented with addin tives, TDZ 1.125 μM and NAA 1.34 μM proved to o be the best combination for high-frequency N multiple shoot induction
  • Spathiflorus, rooting was achieved by IBA treatn ments of shoots for three days, four weeks and o two weeks, respectively, before transferring to the Murashige and Skoog/2 medium-consisting sucrose. e But in B. pallida, well developed shoots when s pulse-treated with IBA 0.5 mg/mL for 30 min u were rooted on the MS half-strength plant l growth hormone medium consisting of both ia sucrose and glucose in 4-5 weeks at 25±1oC and 33.78 μmol photons m-2 s-1 light for c a 12-h photoperiod

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