An analysis of the biofilms adhered to framework alloys using in vitro denture plaque models

Our results showed that the biofilm formation on each framework alloy was different in terms of both the quantity and quality

Yu URUSHIBARA; Tomoko OHSHIMA; Maki SATO; Yoshiko HAYASHI; Tohru HAYAKAWA; Nobuko MAEDA; Chikahiro OHKUBO

2014

Scholarcy highlights

  • The wearing of removable dentures can lead to the colonization of oral pathogenic bacteria and/or poor oral hygiene1-3)
  • Measurement of the surface properties The surface roughness and contact angle of each sample coated with filtered saliva was within the range of surface roughness 0.04–0.05 μm and contact angle 32– 36°, respectively
  • The results of the PCR analysis for the five alloys and the resin sample showed that several microorganisms associated with aspiration pneumonia, including genera of Actinomyces, Fusobacterium, Haemophilus, Mycoplasma and Peptostreptococcus, existed in the biofilms formed on the five alloys and resin
  • We made the following findings: 1. The quantitative analysis revealed that the amount of biofilm with total bacteria from saliva or S. mutans or C. albicans formed on the CP Ti and PGA was almost the same or higher than that formed on the resin surface, while that form on the Ag-Pd-Au was lower than that on the resin
  • The qualitative analysis of the biofilms by terminal restriction fragment length polymorphism suggested that the bacterial components in the biofilms were diverse, and varied widely among the framework alloys
  • There was no significant difference in the contact angle among the all samples
  • Oral microorganisms associated with caries, periodontal disease, aspiration pneumonia and systemic diseases were contained in the biofilms adhered to the framework alloys and resin
  • The quantitative and qualitative properties of the biofilms adhered on the framework alloys were determined

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