Detection and identification of peroxiredoxin 3 as a biomarker in hepatocellular carcinoma by a proteomic approach

The results indicated that overexpression of peroxiredoxin 3 was associated with 94.9% Hepatocellular carcinoma, and correlated with poor differentiation, which suggest that PRDX3 has substantial clinical impact on the progression of hepatocarcinoma, and may be a potential therapeutic target for HCC

Qifa Ye

2012

Scholarcy highlights

  • Hepatocellular carcinoma is one of the most common malignancies worldwide with poor prognosis due to limited efficacy of radiotherapy and resistance to traditional chemotherapy
  • The 2-DE-based proteomic profiling approach was employed in triplicate to analyze the differential expressed proteins in 27 pairs of tumor and the matched non-tumor liver tissues resected from HCC patients who received curative hepatic surgery
  • With the PD-Quest 2D analysis software, statistical analysis of resultant 2-D gels revealed that more than 846 spots were detected in each Coomassie stained gel, and 22 out of 43 protein spots differentially expressed between HCC and adjacent non-tumor tissues were identified by MS/MS analysis
  • The overall expression levels in the tumor tissues were higher than those in the matched non-tumor tissues, which was consistent with the results of the cell lines. These results confirmed that higher levels of peroxiredoxin 3 were expressed in HCC or hepatoma cell lines compared with non-tumor tissues or normal liver cell line at both the mRNA and protein levels
  • 15 were upregulated, whereas the other 7 were downregulated in HCC. They were classified into several functional groups including metabolism, signal transduction, anti-apoptosis, and redox regulation.
  • These proteins could be functionally interrelated to important cellular events, such as cell energy metabolism, antioxidation, cell proliferation, which have been related to the tumorigenesis and progression of malignancies
  • We analyzed the comparative proteomic profiles between Hepatocellular carcinoma tumors and adjacent non-tumor tissues by 2-DE and MALDI-TOF MS, and identified differentially expressed molecules which may help to develop effective therapeutic strategies against HCC

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