Neuroprotective effects of selegiline on rat neural stem cells treated with hydrogen peroxide

The results indicated that the viability of cells pretreated with 20 μM selegiline was significantly increased compared with the control group

Alireza Abdanipour; Iraj Jafari Anarkooli; Saeed Shokri; Mehrdad Ghorbanlou; Vahid Bayati; Reza Nejatbakhsh

2017

Scholarcy highlights

  • The most common neorodegenerative disorders, namely Alzheimer's disease and Parkinson's disease, areKey words: neural stem cells, selegiline, oxidative stress, B‐cell lymphoma 2, heat shock protein 4 prevalent in ~1% of individuals aged 60 years and older
  • Their etiologies remain uncertain, though there are a number of established pathogenic factors, including oxidative stress, neural apoptosis, mitochondrial dysfunction, excitotoxicity, impairment of the ubiquitin‐proteasome system and inflammation
  • The isolated cells were seeded in a 25‐cm2 non‐adherent plastic flask in DMEM/F12 supplemented with 2% B27 supplement, 20 ng/ml basic fibroblast growth factor, 20 ng/ml epidermal growth factor, 100 U/ml penicillin and 100 mg/ml streptomycin and incubated at 37 ̊C in 5% CO2 for 1‐2 weeks to enable neurosphere formation
  • Secondary neurospheres originated from the primary neurospheres, and a homogeneous adherent neural stem cells population was obtained after 3 passages
  • Increased levels of B‐cell lymphoma 2 and Hspa4 mRNA in H2O2‐induced NSCs pretreated with 20 μM selegiline for 48 h were confirmed by reverse transcription‐quantitative polymerase chain reaction
  • In consideration of the increases in Bcl‐2 and Hspa4 mRNA and the potential role of Bcl‐2 in the selegiline‐treated group, the current study indicated that these factors may have been associated with the decreased percentages of necrotic and transferase‐mediated dUTP nick end labeling‐positive cells decreased in the 20 μM selegiline group compared with the 0 and 10 μM groups
  • In consideration of the increases in B‐cell lymphoma 2 and Hspa4 mRNA and the potential role of Bcl‐2 in the selegiline‐treated group, the current study indicated that these factors may have been associated with the decreased percentages of necrotic and transferase‐mediated dUTP nick end labeling‐positive cells decreased in the 20 μM selegiline group compared with the 0 and 10 μM groups

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