Single-Walled Carbon Nanotubes as Enhancing Substrates for PNA-Based Amperometric Genosensors

In recent decades, several novel materials with promising properties for electrochemical sensing have been developed

Simone Fortunati; Andrea Rozzi; Federica Curti; Marco Giannetto; Roberto Corradini; Maria Careri

2019

Scholarcy highlights

  • In recent decades, several novel materials with promising properties for electrochemical sensing have been developed
  • The sequence of the capture probes was complementary to a 20-mer portion of the target DNA; a second biotin-tagged Peptide Nucleic Acid-signalling probe, with sequence complementary to a different contiguous portion of the target biotin-tagged PNA-SP, with sequence complementary to a different contiguous portion of the target
  • The read-out of the electrochemical genosensor assay is carried out using Hydroquinone Diphosphate as enzymatic substrate, which is enzymatically converted to HQ, yielding a voltammetric signal proportional to the substrate, which is enzymatically converted to HQ, yielding a voltammetric signal proportional to amount of PNA-SP hybridized on the electrode surface
  • Concerning intermediate precision, good results were achieved for both the electrode substrates, relative standard deviations being always lower than 10%. Findings of this comparative study demonstrate the outstanding enhancement properties of single-walled carbon nanotubes when used as highly efficient and reactive substrates for covalent immobilization of PNA probes. Efficiency of such nanostructures as electrode materials relies in the improvement of the loading capability of the receptors as well as in the amplification of the electron transfer phenomena involved in the signal transduction mechanism
  • The typical amplification properties of Carbon NanoTubes for electron transfer phenomena are well known and consolidated, as reported in several studies published in the literature, and in this work these advantages were confirmed by our findings in the field of genosensors based on PNA probes
  • The overall performance of the genosensor based on SWCNT-SPEs proved to be undoubtedly superior to the analogous sensing devices implemented on GC-SPEs in many respects, primarily in terms of sensitivity and benefit-cost ratio, paving the way for the exploitation of these systems for development of smart, efficient and portable tools for diagnostic purposes

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