Identification of Angiogenic Cargo in Extracellular Vesicles Secreted from Human Adipose Tissue-Derived Stem Cells and Induction of Angiogenesis In Vitro and In Vivo

These results demonstrate that adipose tissue-derived stem cells -extracellular vesicles contain angiogenic proteins as cargo and promote angiogenesis in vitro and in vivo

Prakash Gangadaran; Ramya Lakshmi Rajendran; Ji Min Oh; Eun Jung Oh; Chae Moon Hong; Ho Yun Chung; Jaetae Lee; Byeong-Cheol Ahn

2021

Scholarcy highlights

  • The morphology of adipose tissue-derived stem cells-extracellular vesicles was observed by Transmission Electron Microscopy, which revealed that the EVs had a typical spherical morphology and were mostly intact
  • The results revealed that interleukin 8, called chemokine ligand 8, was present in ADSC-EVs at a higher concentration than the other proteins, followed by chemokine ligand 2, a tissue inhibitor of metalloproteinases 1, TIMP-2, and vascular endothelial growth factor-D
  • Our results showed that ADSC-EVs induced a more tube-like formation of endothelial cells compared with the control, which was confirmed by AngioTool analysis
  • The results of the current study reveal the presence of proangiogenic proteins in human primary ADSC-EVs, and demonstrate the proangiogenic effect of ADSC-EVs in vitro and in vivo
  • These findings suggest that ADSC-EVs, which are feasibly isolated from adipose tissue, are a promising therapeutic for ischaemic diseases in the future
  • All authors have read and agreed to the published version of the manuscript

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