Knockdown of microRNA-135b in Mammary Carcinoma by Targeted Nanodiamonds: Potentials and Pitfalls of In Vivo Applications

We found NDA135b to target tumor cells, but it binds to granulocytes

Romana Křivohlavá

2019

Scholarcy highlights

  • Breast cancer is one of the most frequent women cancers worldwide and results in 13% of total cancer deaths
  • In order to restore microRNA homeostasis resulting in impairment of tumor growth and its sensitization to conventional therapy, synthetic RNA is introduced into cancer cells using suitable carriers
  • Such synthetic RNA can be designed to target microRNA overexpressed in tumor cells
  • We focus on high-pressure and high-temperature ND with multiple coatings consisting of tumor antigen, polymer link, and sequence-specific antimiR
  • Unbound PEI and Tf were removed by centrifugation and repeated dispersion of the NDs fraction in sterile deionized water in ND concentration 1 mg/mL.-PEI-coated ND were kept in a sonication bath, and immediately incubated for 1 h at room temperature with 270 μg of RNA
  • Our presumption was based on the result mentioned in Figure 3d showing that in total, 4% of macrophages in our MF samples express transferrin receptor
  • We found significantly increased amount of lactate dehydrogenase only in cells stimulated with A135b without difference in carrier
  • Comparing the two different in vivo administrations, the localized intratumoral application was effective in order to knockdown target microRNA-135b and exhibited no leakage of NDA135b into bodily fluids or other tissues

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