Image- and Fluorescence-Based Test Shows Oxidant-Dependent Damages in Red Blood Cells and Enables Screening of Potential Protective Molecules

The present study describes the use of a test ) based on fluorescence and label-free morphology readouts to and quickly evaluate the oxidant and antioxidant properties of various compounds in controlled conditions

Manon Bardyn; Jérôme Allard; David Crettaz; Benjamin Rappaz; Gerardo Turcatti; Jean-Daniel Tissot; Michel Prudent

2021

Scholarcy highlights

  • Red blood cells work in a high O2 content environment and possess a robust system to fight against oxidative stress
  • The imbalance between oxidants and antioxidants that generates this stress is counteracted by chemical-based antioxidant systems, such as lipophilic tocopherols, ubiquinol, carotenoids and flavonoids, hydrophilic ascorbic acid, uric acid and thiols, as well as enzyme-based systems such as glutathione peroxidase or reductase, superoxide dismutase
  • The RBCs were pre-incubated for one hour with AA, UA, trolox or resveratrol antioxidants
  • For RBCs treated with AAPH and diamide, fluorescence emission was reduced proportionally to the amount of antioxidant added
  • The present work investigates the behavior of RBCs under various protective and stress treatments and highlights different cell responses in function of the oxidant and antioxidant properties and their concentrations
  • It is a valuable tool for investigating both the aging of RBCs under various conditions and discovering hit molecules that could be added in blood bags to help better preserve RBCs for transfusion

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