In Vitro Photodynamic Effects of the Inclusion Nanocomplexes of Glucan and Chlorin e6 on Atherogenic Foam Cells

These results suggest that Glu/chlorin e6 nanocomplexes can be a photoactivatable material for treating atherogenic foam cells

Jae Won Ahn; Jin Hyuk Kim; Kyeongsoon Park

2020

Scholarcy highlights

  • Atherosclerosis is a chronic inflammatory disease characterized by the accumulation of lipids in the large arteries
  • We investigated whether Glu/chlorin e6 nanocomplexes are taken up by both macrophages and foam cells, using a customized multi-channel confocal laser scanning microscope
  • We investigated whether Glucan and and Chlorin e6 nanocomplexes are taken up by both on macro
  • We performed blocking experiments by pretreating the foam cells with free laminarin and free Glu to confirm whether the cellular uptake of Glu/Ce6 nanocomplexes occurs via dectin-1 receptor-mediated endocytosis
  • We have limitation for dectin-1 binding affinity of Glu/Ce6 nanocomplexes on foam cells, these results suggest that Glu/Ce6 nanocomplexes deliver Ce6 molecules into foam cells with a better efficacy compared to normal macrophages
  • To demonstrate whether Glu/Ce6 nanocomplexes induce the apoptosis of foam cells compared to free Ce6, an annexin V/propidium iodide stain experiment indicating that the membranes of foam cells were more photo-damaged, as compared to was performed
  • Laser treatment significantly damaged the foam cell membranes and increased cell apoptosis

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