Isoproterenol-Induced Permeability Transition Pore-Related Dysfunction of Heart Mitochondria Is Attenuated by Astaxanthin

Based on the data obtained, we suggest that AST has a protective effect in rat heart mitochondria

Roman Krestinin; Yulia Baburina; Irina Odinokova; Alexey Kruglov; Irina Fadeeva; Alena Zvyagina; Linda Sotnikova; Olga Krestinina

2020

Scholarcy highlights

  • Mitochondria are the center of cellular energy production and a major source of reactive oxygen species
  • Because mitochondrial dysfunction induced by oxidative stress can affect the functional state in mitochondria, we examined the effects of mitochondrial permeability transition pore opening induced in rat heart mitochondria by the administration of AST and injection of ISO on the membrane potential, and the Ca2+ retention capacity in RHM isolated from each group of rats
  • It is known that regulatory proteins play an important role in the mPTP; we examined how the administration of AST and ISO influences the concentrations of proteins such as adenine nucleotide translocase, voltage-dependent anion channel, subunit c, cyclic nucleotide -phosphodiesterase, and subunit b of ATP synthase in RHM isolated from each group of rats
  • We found that the level of CNPase diminished in RHM isolated from ISO-injected rats, whereas the application of AST in combination with ISO increased the content of CNPase when the mPTP was closed/open
  • The administration of AST in combination with ISO increased the half-maximum value of mitochondrial swelling by 40% in comparison with the control
  • AST administration accelerated ROS production; it prevented the elimination of CL, which plays an important role in the regulation of membrane integrity and the activity of respiratory chain complexes
  • All of the aforesaid results suggest that AST exhibits protective properties in mitochondria and can be used to prevent the development of cardiovascular diseases

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