PbGA20ox2 Regulates Fruit Set and Induces Parthenocarpy by Enhancing GA4 Content

Our study revealed that PbGA20ox2 altered the GA biosynthetic pathway and enhanced GA4 synthesis, thereby promoting fruit set and parthenocarpic fruit development

Huibin Wang; Ting Wu; Jianlong Liu; Liu Cong; Yanfei Zhu; Rui Zhai; Chengquan Yang; Zhigang Wang; Fengwang Ma; Lingfei Xu

2020

Scholarcy highlights

  • Fruit set, a key developmental process that occurs following successful fertilization in higher plants, is the first step in fruit development
  • We found that GA4+7 induced seedless pear fruit; to investigate the mechanism by which GA induces parthenocarpy in pear, we analyzed the RNAseq data, which showed that pollination induced the expression of gibberellin biosynthesis pathway related genes, such as, PbGA3ox, Ent-Kaurene Synthase, Ent-Kaurene Oxidase, and PbGA20ox genes, and the expression of PbGA20ox2 was increased dramatically
  • GA 20-oxidases, as a rate-limiting enzyme, plays a key role in the GA synthesis pathway, so we inferred that it may be related to fruit development
  • On the basis of conserved domains of the GA20ox subfamily, we identified three PbGA20ox genes in pear, PbGA20ox1, PbGA20ox2, and PbGA20ox3
  • PbGA20ox1 and PbGA20ox3 gene expression levels were reduced in pollinated fruits at 9 DAA; their expression levels after pollination were lower than in fruit treated with exogenous GA at 14 DAA, the expression of PbGA20ox3 increased at 3 DAA
  • We demonstrated that PbGA20ox2 plays key roles in fruit set and development
  • An analysis of endogenous GAs levels in transgenic tomato and pear revealed that PbGA20ox2 promoted fruit set and development by increasing the accumulation of GA4 at an early stage of fruit development

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