Overexpression of Jatropha Gibberellin 2-oxidase 6 (JcGA2ox6) Induces Dwarfism and Smaller Leaves, Flowers and Fruits in Arabidopsis and Jatropha

The results indicated that overexpression of JcGA2ox6 had a great impact on the vegetative and reproductive growth of transgenic Jatropha

Ying-Xiong Hu; Yan-Bin Tao; Zeng-Fu Xu

2017

Scholarcy highlights

  • Physic nut is a perennial woody plant that belongs to the Euphorbiaceae family and is monoecious, with male and female flowers borne on the same inflorescence
  • When WT and transgenic plants were grown in the field for approximately 3 months, inflorescence buds emerged in both plants, suggesting that the flowering time of Jatropha was not affected by overexpression of JcGA2ox6, unlike the late-flowering noted in transgenic Arabidopsis
  • The transgenic Arabidopsis and Jatropha overexpressing JcGA2ox6 demonstrated the GA-deficient phenotype of dwarfism
  • By analyzing the contents of the endogenous GAs in transgenic Jatropha seedlings, the levels of bioactive GA4 significantly decreased in transgenic lines and were correlated with the degree of dwarfism
  • The bioactive GA1 and GA7 were not detected in either the WT or transgenic lines, suggesting that they did not function as a promoter in the stem elongation of Jatropha seedlings, not to mention GA3 which was elevated in the transgenic lines
  • The transgenic lines bolted later under both LD and SD conditions, but there was no significant difference in rosette leaf number
  • Perhaps GA1, GA3 and GA7 are required in other aspects of Jatropha growth and development, as the major bioactive GA is different in the diverse tissue development stages of some plant species
  • Overexpression of JcGA2ox6 delayed the flowering time in transgenic Arabidopsis with no significant difference in the number of rosette leaves

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