Genome-wide identification and expression analysis of auxin response factor gene family in Medicago truncatula

Our results show that changes in MtARF gene expression occur during the response to S. meliloti infection, suggesting that members of this family may have important roles in the symbiotic interaction

Chenjia Shen; Runqing Yue; Tao Sun; Lei Zhang; Luqin Xu; Shuanggui Tie; Huizhong Wang; Yanjun Yang


Scholarcy highlights

  • The phytohormone auxin is involved in regulating many aspects of plant growth and development, including root system architecture re-establishment under nutrient stress and responses to environmental stimuli
  • Expression and functional characterization of the Auxin Response Factor are needed if the mechanisms behind how auxin is involved in plant growth and response to environmental stimuli in a spatio-temporal specific manner are to be revealed
  • Focusing on the main structural and expression features of ARF genes in M. truncatula, which is a model indeterminate legume, helped us to describe the relationship between ARF-mediated auxin signaling and the symbiotic association with nitrogen-fixing bacteria during the early phase of S. meliloti infection
  • The Carboxy-Terminal Domains, which is involved in homoand hetero-interactions among ARFs, resembles the III and IV domain of Aux/indole-3-acetic acid proteins
  • The presence of a large number of CTD-truncated MtARFs suggested that many auxin-responsive genes in M. truncatula can be regulated in an auxin independent manner
  • The activator/repressor ratio among ARFs in M. truncatula was only 0.26, which was similar to tomato and nearly half of that in Arabidopsis or rice
  • S. meliloti infection may first affect the expression levels of Auxin Response Factor family genes to help nodule formation and differential expressions of MtARF genes may be involved in the resistant phenotype of dmi3 mutant

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