Skin Mucus of Gilthead Sea Bream (Sparus aurata L.). Protein Mapping and Regulation in Chronically Stressed Fish

By using our gilthead sea bream protein database we identified 2,466 spectra with a much higher protein score

Jaume Pérez-Sánchez; Genciana Terova; Paula Simó-Mirabet; Simona Rimoldi; Ole Folkedal; Josep A. Calduch-Giner; Rolf E. Olsen; Ariadna Sitjà-Bobadilla


Scholarcy highlights

  • A keratinized multi-sheet cellular layer covers the epidermis of amphibian adults, reptiles, birds and mammals, whereas skin mucus constitutes the outermost epidermal barrier in fish and aquatic amphibian larvae
  • Several cell types are involved in regulating the composition of the skin mucus layer, it is mainly shaped by Goblet cells that release mucous granules containing high molecular weight glycoproteins called mucins
  • These O-glycosylated glycoproteins are present on the apex of all wet-surfaced epithelia with a well-defined expression pattern, which can be disrupted in response to a wide range of injuries or challenges
  • The second node of interconnected skin proteins was composed of 79 proteins involved in protein ubiquitination and antigen presentation pathways with a high representation of major histocompatibility complex, proteasome subunits, ubiquitin enzymes and molecular chaperones, including calnexin, calreticulin and heat shock proteins representative of the six major HSP families based on molecular mass with either cytoplasmic, nuclear plasma membrane or extracellular locations. This agrees with the observations made in a previous proteomic gilthead sea bream study, in which more than 1,300 spots were recorded in the skin mucus, but the 100 most abundant were among others ubiquitin/proteasome-related proteins and HSPs
  • Three major clusters with more than 350 proteins were retained after filtering by canonical pathway overlapping
  • Proteins of oxidative phosphorylation, mitochondrial dysfunction, protein ubiquitination, immune response, epithelial remodeling, and cellular assembly were highly represented. This was reinforced by the observation that major changes related to the abundance of cytokeratin 8 in the skin mucus of stressed fish under our experimental model of chronic stress were found by means of 2-DE methodology and confirmed by immunoblotting
  • A high resolution mass spectrometry-based proteomic approach was able to identify 2,062 proteins in the skin mucus of gilthead bream after matching in a homologous protein database

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