The Change of Teleost Skin Commensal Microbiota Is Associated With Skin Mucosal Transcriptomic Responses During Parasitic Infection by Ichthyophthirius multifillis

We found that Ichthyophthirius multifiliis infection led to a decreased abundance of skin commensals and increased colonization of opportunistic bacteria through 16S rRNA pyrosequencing, which were mainly characterized by lose of Proteobacteria and increased intensity of Flavobacteriaceae

Xiaoting Zhang; Liguo Ding; Yongyao Yu; Weiguang Kong; Yaxing Yin; Zhenyu Huang; Xuezhen Zhang; Zhen Xu

2018

Scholarcy highlights

  • The skin of vertebrates serve as the first line of defense against pathogens’ invading and harbor millions of microorganisms that form a very ancient and successful symbiosis between prokaryotes and metazoans
  • These results firstly demonstrate that the skin mucosal immune responses are associated with changed commensal microbiota after parasitic infection in teleost fish
  • We detected the expression of Ichthyophthirius multifiliis-18SrRNA in skin of trout after Ich infection and that of the control by quantitative real-time PCR
  • Some protozoan parasite disease pathways were significantly enriched in the E7d/Con group, including amoebiasis and malaria. These results further suggest that the differential expression genes’ of the complement system play a crucial part in defense against parasite invasion and that a stronger immune response to Ich had occurred in the trout skin of E7d/Con group
  • We calculated the differences in the microbial diversity and community in trout skin between the experimental and control groups by using the operational taxonomic units noted above for further analysis with UniFrac
  • We found significantly increased abundances of Flavobacterium, Rickettsiaceae, and Bdellovibrio at 7 d after infection
  • High alpha diversity values of microbiota in trout skin from the experimental groups were discovered at both 24 h and 7 d after Ich infection when compared with the control group
  • Future studies should address how the defensive mechanisms of specific immune responses in trout skin against Ichthyophthirius multifiliis infection correlate with the observed changes in the skin microbiome of teleost fish

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