Effects of Experimental Terrestrialization on the Skin Mucus Proteome of African Lungfish (Protopterus dolloi)

Using LC-MS/ MS, we identified a total of 974 proteins using a lungfish Illumina RNA-seq database, 1,256 proteins from previously published lungfish sequence read archive and 880 proteins using a lungfish 454 RNA-seq database for annotation in the three samples analyzed

Ryan D. Heimroth; Elisa Casadei; Irene Salinas


Scholarcy highlights

  • Transitioning to life on land was a fundamental step in the success and diversification of the vertebrate lineage
  • In aquatic vertebrates, such as teleost fish, the skin is a mucosal epithelium composed of layers of living cells coated by a mucus layer that is in direct contact with the environment
  • Histological examination of freshwater and terrestrialized P. dolloi skin revealed that mucus-secreting goblet cells become exhausted from the process of terrestrialization
  • We observed eosinophilic granulocytes in the dermis of both samples, but they were more abundant in the terrestrialized skin
  • A similar trend was observed when using the 454 translated transcriptome with 33 shared proteins among all three mucus samples, 130 proteins unique to the free-swimming skin mucus, 101 proteins unique to the terrestrialized skin mucus, and only 14 proteins unique to the terrestrialized gill mucus. These results suggest that the composition of the gill mucus secretion produced during the induction phase of terrestrialization resembles both the free-swimming skin mucus and the terrestrialized skin mucus proteome
  • This study provides the first characterization of the skin mucus proteome of a sarcopterygian fish, the African lungfish
  • We report important shifts in both innate and adaptive immune molecules

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