Non-esterified Fatty Acid Induce Dairy Cow Hepatocytes Apoptosis via the Mitochondria-Mediated ROS-JNK/ERK Signaling Pathway

The results indicated that non-esterified fatty acid inhibited hepatocytes growth and increased LDH release from hepatocytes in a dose-dependent manner

Yu Li; Hongyan Ding; Leihong Liu; Yuxiang Song; Xiliang Du; Shibin Feng; Xichun Wang; Xiaobing Li; Zhe Wang; Xinwei Li; Jinchun Li; Jinjie Wu; Guowen Liu

2020

Scholarcy highlights

  • In dairy cows, ketosis is a metabolic disorder that occurs primarily during the transition period
  • The results indicated that non-esterified fatty acid inhibited hepatocytes growth and increased LDH release from hepatocytes in a dose-dependent manner
  • We found that NEFA enhanced MDA production, which markedly increased in the 0.6, 1.2, and 2.4 mM NEFA treatment groups
  • The intracellular reactive oxygen species levels in hepatocytes were analyzed via DCFH-DA staining, revealing a NEFA dose-dependent increase in intracellular ROS levels, which were significantly higher in the 0.3, 0.6, 1.2, and 2.4 mM NEFA treatment groups compared with the control group
  • These results indicated that NEFA induces apoptosis in hepatocytes via a ROS-dependent pathway
  • After a 4-h attachment period, the medium and unattached cells were removed and replaced with growth medium containing 5% fetal bovine serum
  • The results of this study demonstrated that NEFA trigger hepatocytes apoptosis via the Jun N-terminal kinase/ERK activation-mediated mitochondria-dependent apoptosis pathway, including the transcriptional activation of p53, the transcriptional inhibition of nuclear factor E2-related factor 2, a loss of the membrane potential, the cleavage of caspase 3 and PARP and the translocation of apoptosis-inducing factor and cyt c as crucial events involved in apoptosis
  • Excessive oxidative stress signals initiate hepatocytes apoptosis, causing liver damage

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