Diclofenac-induced cytotoxicity in cultured carp leukocytes

Our study aimed to investigate the response of cultured carp leukocytes following exposure to diclofenac, a frequent aquatic environment contaminant around the world

M Nemcova; J Pikula; J Zukal; V Seidlova

2021

Scholarcy highlights

  • Human and wildlife health and disease are intricately interlinked
  • Summary Diclofenac is a drug commonly used in human and veterinary medicine for the treatment of diseases associated with inflammation and pain
  • Cytotoxic and -static effects of diclofenac in concentrations of 0.001 μg/ml, 0.01 μg/ml, 0.1 μg/ml, 1 μg/ml, 10 μg/ml and 100 μg/ml for the carp cultured leukocytes were quantified using detection of lactate dehydrogenase released from damaged cells
  • Leukocyte viability is essential for immune functions and any change can lead to reduction of resistance against pathogens, mainly in cold year seasons, when the immune system is naturally suppressed
  • Following chemicals were used during cultivation and testing: culture medium Dulbecco's Modified Eagle Medium/Ham's F-12, assay medium Dulbecco's Modified Eagle Medium w/o sodium pyruvate, bovine calf serum 10 %, antibiotics, diclofenac sodium salt, 1A2 Cytochrome P450 human expressed in Pichia pastoris, lactate-dehydrogenase activity assay kit, dimethyl sulfoxide, phosphate saline buffer, and trypsin solution
  • Experiment 1 The longest incubation period was excluded from the analysis because cytotoxicity results were extremely influenced by the increasing inhibition of cell growth. This negative correlation was significant at all diclofenac concentrations except for 0.1 μg/ml diclofenac cytotoxicity was generally low and does not occur within short periods of cell incubation and exposure

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