Methylene blue elicits non-genotoxic H2O2 production and protects brain mitochondria from rotenone toxicity

We investigated the dose-dependent H2O2 production in intact brain mitochondria and showed the increase in the H2O2 production after adding as little as 50 nM Methylene blue

Artem P. Gureev; Ekaterina A. Shaforostova; Denis A. Laver; Victoria G. Khorolskaya; Mikhail Yu. Syromyatnikov; Vasily N. Popov

2019

Scholarcy highlights

  • Methylene blue is an organic basic thiazine dye widely used as a medicine for malaria, methemoglobinemia and cyanide poisoning
  • The rate of H2O2 generation MB increases the rate of H2O2 production. 50 nM MB caused its increase to 585 ± 36 pmol/min/mg of protein in mitochondria respirated on 5 mM malate and 5 mM glutamate
  • Summarizing the data obtained in this research, we can conclude that MB increases production of H2O2 due to bypassing of ETC components of brain mitochondria
  • MB contributes to activation of mitochondrial biogenesis through Nrf2 by stimulation of H2O2 production
  • At the same time MB-related H2O2 was produced in non-toxic volume that does not exert genotoxic effect on mtDNA, which is the most susceptible to oxidative damage
  • All above mentioned proves the possibility of using mild oxidative stress in the activation of compensatory reactions, which may have a therapeutic effect in the treatment of neurodegenerative age-depended diseases

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