Embedding in Epoxy Resins for Ultrathin Sectioning in Electron Microscopy

Fixed tissue is dehydrated with tertiary butyl alcohol overnight

K. C. Richardson; L. Jarett; E. H. Finke

2007

Scholarcy highlights

  • Fixed tissue is dehydrated with tertiary butyl alcohol overnight
  • The following day it is cleared in toluene, infiltrated and embedded in Araldite resin-hardener-accelerator mixture without dibutyl phthalate, and polymerized at 60° C
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