Detecting translocation of individual single stranded DNA homopolymers through a fabricated nanopore chip

Based on the analysis of the current blockage caused by DNA translocation through a voltage-biased nanopore, we discovered that the hydrodynamic diameter of single stranded DNA homopolymer helix is comparable to that of double stranded DNA helix

Young-Rok Kim

2007

Scholarcy highlights

  • Fabricated solid-state nanopore chips are used to probe individual single stranded DNA homopolymers
  • Based on the analysis of the current blockage caused by DNA translocation through a voltage-biased nanopore, we discovered that the hydrodynamic diameter of single stranded DNA homopolymer helix is comparable to that of double stranded DNA helix
  • This proof-of-principle demonstration shows that solid-state nanopore technology can be used to spy on secondary structures of biopolymers
  • We show that ssDNA manifests slower and distinct translocation kinetics in comparison to double stranded DNA
  • The present study helps to refine our understanding of the ssDNA translocation kinetics through narrower alpha-hemolysin protein pores

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