Differential expression analysis of boron transporters and some stress-related genes in response to 24-epibrassinolide and boron by semi-quantitative RT-PCR in Arabidopsis thaliana (L.) Heynh

To examine the possible role of BR in high B-induced stress at the transcriptional level, we investigated the response of B transporter genes, high B-induced genes, BR-induced genes and other stress-related genes ) upon exogenous treatments of 24-epibrassinolide on Arabidopsis thaliana Heynh exposed to high concentrations of boric acid using semi-quantitative reverse transcriptase -polymerase chain reaction

Yonca Surgun; Bekir Çöl; Betül Bürün

2016

Scholarcy highlights

  • Boron is an essential micronutrient required by the plants
  • To examine the possible role of BR in high B-induced stress at the transcriptional level, we investigated the response of B transporter genes, high B-induced genes, BR-induced genes and other stress-related genes) upon exogenous treatments of 24-epibrassinolide on Arabidopsis thaliana Heynh exposed to high concentrations of boric acid using semi-quantitative reverse transcriptase-polymerase chain reaction
  • Expression patterns of the genes were monitored by semi-quantitative RT-PCR in the leaf and root tissues of A. thaliana seedlings exposed to different concentrations of BA and/or EBL
  • Another study revealed that application of high levels of BA represses the expression of aquaporins and nutrient transport genes in the Arabidopsis roots
  • BOR3 exhibits the same pattern of expression as BOR1 both in leaf and root tissues in response to BA treatments
  • It was proposed that the function of BOR2 is to transport BA from symplast to apoplast for cross-linking of B to Rhamnogalacturonan II in cell wall
  • The increase in the transcript levels of abscisic acid-responsive genes) in EBLtreated tissues suggests that stress tolerance provided by BRs may occur through the interaction present in the pathways of the phytohormones such as ABA, ethylene and salicylic acid

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