Functional Analysis of Multiple nifB Genes of Paenibacillus Strains in Synthesis of Mo-, Fe- and V-Nitrogenase

We demonstrated that the transcriptions of nifB being adjacent to nif or anf or vnf genes significantly expressed under N2-fixation conditions and are active in synthesis of Mo-, Fe and V-nitrogenase

Qin Li; Haowei Zhang; Liqun Zhang; San-Feng Chen

2021

Scholarcy highlights

  • Biological nitrogen fixation is catalyzed by Mo, V- and Fe-nitrogenases that are encoded by nif, vnf and anf genes, respectively
  • We demonstrated that the transcriptions of nifB being adjacent to nif or anf or vnf genes significantly expressed under N2-fixation conditions and are active in synthesis of Mo, Fe and V-nitrogenase
  • The nitrogen fixation genes in the genomes of the 116 diazotrophic Paenibacillus strains taken from the RefSeq database were comparatively analyzed
  • A total of 138 NifB putative sequences were found in the 116 diazotrophic Paenibacillus strains
  • In order to investigate whether the nifB1 and nifB2 from P. forsythia T98 and P. zanthoxyli JH29 were active in synthesis of Fe-nitrogenase and V-nitrogenases, the ΔnifBHDK and ΔnifBHDKEN mutants of P. polymyxa WLY78 which lost the ability to synthesize Mo-nitrogenase were constructed
  • The results indicated that the reconstituted vnf system composed of 10 genes can support synthesis of V-nitrogenase to fix nitrogen
  • Our study provides guidance for engineering nitrogen fixation genes into heterologous hosts for nitrogen fixation

Need more features? Save interactive summary cards to your Scholarcy Library.