Preparation and characterization of carboxyl-group functionalized superparamagnetic nanoparticles and t he potential for bio-applications

The nanoparticles were employed to isolate plasmid DNA from bacterial cell culture and the results demonstrated its applicability in DNA preparation

Zhi Shan

2008

Scholarcy highlights

  • In recent years there has been a great deal of interest with the rapid development of nanostructured materials and nanotechnology in the fields of biomedicine, including drug targeting, MRI diagnosis, hyperthermia treatment of cancers and biotechnology that include detection and separation of cells, biomolecules such as proteins and nucleic acids. Magnetite and maghemite have been widely used in these cases because they are nontoxic, and with magnetic properties. It is known that when the particle size is smaller than 30 nm, magnetite and maghemite particles display superparamagnetic properties, i.e., they are attracted to a magnetic field but retain no magnetism after removal of magnet. This property facilitates the magnetically drivenPreparation and Characterization of Carboxyl-GroupJ
  • A portion of the washed magnetic nanoparticles was dried for subsequent characterization using the same dry process as described for the uncoated iron oxide
  • After immobilization of the particles, the supernatant was removed by discarding and evaporating and the plasmid DNA desorbed by addition of 50 μL of elution buffer at room temperature for 1 min
  • It was noticed that the color of unmodified nanocrystals slowly changed from black to brown when exposed to air at room temperature before subsequent coating work and characterization
  • A typical transmission electron microscope image for the surface modified magnetic nanoparticles is shown in Figure 2
  • Maghemite nanocrystals were prepared by quick-pour-precipitation method and these superparamagnetic crystals were modified with carboxyl groups by coating with functional agent Methacrylic acid
  • The carboxyl moiety on the particle surface provided a binding site for the chemical immobilization of various molecules for potential specific molecular recognition

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