The Bradford Method for Protein Quantitation

Reade, S.M. and Northcote, D.H. Minimization of variation in the response to different proteins of the Coomassie Blue G dye-binding assay for protein

John B. W. Hammond; Nicholas J. Kruger

2003

Scholarcy highlights

  • Reade, S.M. and Northcote, D.H. Minimization of variation in the response to different proteins of the Coomassie Blue G dye-binding assay for protein
  • A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study
  • The Lowry method has been widely used, but is susceptible to interference from a wide range of compounds commonly present in biological extracts
  • Interference can be avoided by trichloracetic acid precipitation of the protein prior to assay, this lengthens the procedure
  • (1981) Minimization of variation in the response to different proteins of the Coomassie Blue G dye-binding assay for protein

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