Induction of ethylene inhibits development of soybean sudden death syndrome by inducing defense-related genes and reducing Fusarium virguliforme growth

Our results suggest that ethephon application inhibits Sudden death syndrome development directly by slowing Fusarium virguiforme growth and/or by inducing soybean ethylene signaling and the expression of defense related genes

Noor A. Abdelsamad; Gustavo C. MacIntosh; Leonor F. S. Leandro

2019

Scholarcy highlights

  • Sudden death syndrome, caused by the soilborne fungus Fusarium virguiforme is one of the most damaging diseases to soybean production in North and South America
  • 21-day-old cultures on potato dextrose agar were flooded with 20 ml of sterile distilled water, the conidia were dislodged with a rubber policeman, and the suspension was filtered through a double layer of sterile cheesecloth
  • Fusarium virguliforme infection triggers ethylene biosynthesis in soybean To examine whether Fv inoculation could induce ethylene biosynthesis in SDS resistance and susceptible soybean cultivars, the expression of key ethylene biosynthesis genes, 1-aminocyclopropane-1-carboxylic acid synthase, and 1-aminocyclopropane-1-carboxylic acid oxidase, were quantified in roots at 2 and 4 days post inoculation and compared to time 0
  • These results suggest that ethylene biosynthesis might be important in resistance against SDS
  • Drench applications of ethephon at 4mM enhanced the expression of genes involved in soybean ethylene biosynthesis and defense responses. These results suggest that ethephon soil application is a promising inducer of ethylene signaling pathway that may play a positive role in soybean resistance against Fv infection
  • There were no significant differences in root rot severity between chemical treatments and the water control
  • A limitation of our study is that ethephon was applied to soybean seedlings at VC stage, before Fv infection, and under controlled environmental conditions
  • If ethylene is shown to enhance resistance against Fusarium virguiforme infection in field conditions, transcriptomic analysis of soybean seedlings in response to ethephon treatment is needed to identify resistant genes that could be incorporated into breeding against Fv. S1 Fig. Expression analysis of 1-aminocyclopropane-1-carboxylic acid synthase gene in response to Fusarium virguliforme infection at different soybean growth stages

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