The Effects of Disturbance on Hypothalamus-Pituitary-Thyroid (HPT) Axis in Zebrafish Larvae after Exposure to DEHP

All the results demonstrated that exposure to Di-(2-ethylhexyl) phthalate altered the whole-body thyroid hormones in the zebrafish larvae and changed the expression profiling of key genes related to HPT axis, proving that DEHP induced the thyroid endocrine toxicity and potentially affected the synthesis, regulation and action of thyroid hormones

Pan-Pan Jia; Yan-Bo Ma; Chun-Jiao Lu; Zakaria Mirza; Wei Zhang; Yong-Fang Jia; Wei-Guo Li; De-Sheng Pei


Scholarcy highlights

  • Phthalates acid esters as plasticizers are wildly used in different kinds of plastic products, such as food contact materials, blood bags, toys, clothing, adhesives, plastic coating, PLOS ONE | DOI:10.1371/journal.pone.0155762 May 25, 2016HPT Toxicity of Di-(2-ethylhexyl) phthalateDistrict, Chongqing, the Scientific Research Starting Foundation for Returned Overseas Chinese Scholars from Ministry of Education
  • It is important to investigate the molecular toxicology of DEHP because of its high concentration found in the environment
  • We used zebrafish embryos and larvae as the model to evaluate the mechanism of thyroid endocrine disruption caused by DEHP
  • After exposure to DEHP, the whole-body thyroid hormones levels and the mRNA expression of key genes related to HPT axis were systematically investigated
  • The results showed that DEHP changed the Thyroid hormones levels and genes expression of HPT axis, suggesting the disturbance effects of DEHP in zebrafish embryos/larvae
  • While pax8 has no significant difference between the exposure and control groups
  • We found that the transcripts of crh and tsh╬▓ gene were significantly up-regulated after DEHP exposure, indicating that DEHP activated the HPT axis
  • This study showed that exposure to Di-(2-ethylhexyl) phthalate altered the whole-body Thyroid hormones levels and the expression of HPT axis related genes, proving the DEHP toxicity on thyroid endocrine in zebrafish larvae

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