Adrenomedullin as an Autocrine/Paracrine Apoptosis Survival Factor for Rat Endothelial Cells*

We demonstrate here a novel role of this peptide as an apoptosis survival factor for rat endothelial cells

Hiroki Kato


Scholarcy highlights

  • APOPTOSIS, a genetically controlled response to eliminate unwanted cells, is involved in the regulation of cell number in several physiological and pathological conditions
  • Cultured human endothelial cells have been demonstrated to undergo apoptosis when detached from culture plates
  • When medium was deprived of serum, rat endothelial cell cultures always contained a fraction of floating cells; almost agarose gel
  • Apoptosis of endothelial cells after serum deprivation was supported by two lines of evidence
  • Floating dead cells had morphological and biochemical features characteristic of apoptosis, such as membrane blebbing, cellular shrinkage, nuclear condensation and fragmentation, and nucleosomal laddering on agarose gel electrophoresis
  • The intra- and interassay variations were less than 10%
  • Adrenomedullin prevented apoptosis as early as 1 h after serum deprivation, whose effect was time-dependent during 24-h incubation period
  • The present study revealed that rat endothelial cells that remained flattened shape and firmly attached to culture plates, exhibited distinct morphological and biochemical evidence of apoptosis, arguing against the involvement of the inhibition of anchorage-dependent cell spreading

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