Presented in part at the 19th Annual Meeting of the Society for Neuroscience, Phoenix, AZ, 1989
In this study we investigated the possibility that preproNPY messenger RNA content may be altered in obese Zucker rats compared to that of their lean littermates
The results revealed a 2- to 3-fold increase in preproNPY messenger RNA levels in the hypothalamus of obese animals compared to lean
An additional RNase protection study was pursued to investigate the effects of 72 h food deprivation on hypothalamic preproNPY mRNA levels in lean and obese animals
Lean animals displayed an approximate 2-fold increase in preproNPY mRNA content, whereas obese animals showed no significant increase after food deprivation
These data are consistent with the hypothesis that Neuropeptide Y projections within the hypothalamus are involved in regulating feeding behavior and weight gain, and that disturbed regulation of hypothalamic NPY expression may play a role in the etiology of obesity in the genetically obese Zucker rat.
Agouti-Related Peptide, Neuropeptide Y, and Somatostatin-Producing Neurons Are Targets for Ghrelin Actions in the Rat Hypothalamus