Localization of Thyrotropin-Releasing Hormone Prohormone Messenger Ribonucleic Acid in Rat Brain by in Situ Hybridization*

We studied the distribution of pro-TRH mRNA in rat brain by in situ hybridization histochemistry using radiolabeled single stranded cRNA probes to confirm the hypothesis that the TRH precursor is distributed beyond regions that contain immunoreactive TRH

THOMAS P. SEGERSON; HEINZ HOEFLER; HENRY CHILDERS; HUBERT J. WOLFE; PING WU; IVOR M. D. JACKSON; RONALD M. LECHAN

2009

Scholarcy highlights

  • We studied the distribution of pro-TRH mRNA in rat brain by in situ hybridization histochemistry using radiolabeled single stranded cRNA probes to confirm the hypothesis that the TRH precursor is distributed beyond regions that contain immunoreactive TRH
  • A single hybridizing band was observed on Northern blots of RNA extracts of the periaqueductal gray and reticular nucleus, identical to that seen in extracts of the paraventricular nucleus
  • The appearance of pro-TRH mRNA in neurons not previously recognized to contain TRH but which contain the prohormone suggests that non-TRH peptides within the TRH precursor may be preferentially expressed in certain regions of the brain.
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