The enriched pathways and highlighted genes in this study revealed novel disruptions in cell signaling cascades which were confirmed by real time-PCR
2021
Comprehensive high-throughput meta-analysis of differentially expressed microRNAs in transcriptomic datasets reveals significant disruption of MAPK/JNK signal transduction pathway in Adult T-cell leukemia/lymphoma
Key concepts
- Adult T cell leukemia
- gene expression
- CD4 + T cell
- signal transduction pathway
- peripheral blood
- lymphoma
- JNK pathway
- Graft-versus-host disease
- leukemia
- MAPK
- Amyotrophic lateral sclerosis
- Human T-lymphotropic virus 1
- Kyoto Encyclopedia of Genes and Genomes
- Gene expression analysis
- HTLV-1
- biology
- Systems biology
- pathophysiology
Scholarcy highlights
- Human T lymphotropic virus 1 is a singlestranded positive-strand RNA virus, which primarily infects CD4+ T-cells in humans
- Further analysis revealed the first 5 of differentially expressed miRNAs were directly associated with previously identified pathways in the pathogenesis of Human T-lymphotropic virus 1
- Network analysis demonstrated the involvement of target gene hubs in several signaling cascades, mainly in the MAPK pathway
- After removal of redundancy, application of inclusion and exclusion criteria, and quality control using MetaQC package in R, 3 datasets namely, GSE28626, GSE31629, and GSE11577 were selected for the DEM analysis of Adult T-cell leukemia/lymphoma patients vs healthy individuals
- The primary analysis of microarray datasets identified hsa-let-7a, hsa-let-7g, hsa-mir-181b, hsa-mir-26b, hsamir-30c, hsa-mir-186, hsa-mir-10a, hsa-mir-30b, and hsa-let-7f as DEMs in ATLL patients compared to normal individuals
- Therapeutic targeting of JNK pathway proves to be an interesting topic for future studies. In this high throughput meta-analysis, we identified significant disruption of genes related to cell cycle, proliferation, and signal transduction in ATLL
- Further studies are needed to identify novel and reliable biomarkers, and prognostic factors and to obtain a comprehensive mapping of deregulated biological pathways in Adult T-cell leukemia/lymphoma
Need more features? Save interactive summary cards to your Scholarcy Library.