An improved strategy to analyse strigolactones in complex sample matrices using UHPLC–MS/MS

We show that the method can be applied to a variety of plant species

Kristýna Floková; Mahdere Shimels; Beatriz Andreo Jimenez; Nicoletta Bardaro; Miroslav Strnad; Ondřej Novák; Harro J. Bouwmeester


Scholarcy highlights

  • Strigolactones represent the most recently described group of plant hormones involved in many aspects of plant growth regulation
  • A broad range of mono- and dicotyledonous, and lower plant species have been shown to produce a blend of SLs in different quantities and combinations of which the production is upregulated by phosphate and nitrogen deficiency
  • Strigolactone stability in solvents One of the first and most critical steps in quantitative analysis of compounds with limited stability is the selection of appropriate solvents
  • We present a highly sensitive and validated UHPLC–MS/MS method for simultaneous profiling of seven naturally occurring canonical strigolactones in some of the most frequently studied plant materials—root tissue and exudates
  • In order to minimize the strong matrix effect, the sample size of root exudate extracts and roots was reduced to 20 ml and 150 mg, respectively
  • For targeted UHPLC–MS/MS analysis a method was developed in which a range of different SLs including their biosynthetic precursors can be separated within a 10 min retention time frame using an analytical column packed with sub-2-microne particles
  • Seven SLs were successfully detected and quantified in a series of very different, real plant-derived samples showing the versatility of the developed method

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