Functional analysis of multiple nifB genes of Paenibacillus strains in synthesis of Mo-, Fe- and V-nitrogenases

We demonstrate that only nifBI and nifBII are functional in synthesis of Mo-, Fe- and V-nitrogenase

Qin Li; Haowei Zhang; Liqun Zhang; Sanfeng Chen

2021

Scholarcy highlights

  • Biological nitrogen fixation is catalyzed by Mo, V- and Fe-nitrogenases that are encoded by nif, vnf and anf genes, respectively
  • Classification of nifB genes of Paenibacillus genus Here, the nitrogen fixation genes in the genomes of the 116 diazotrophic Paenibacillus strains taken from the RefSeq database are comparatively analyzed
  • A total of 138 NifB putative sequences are found in the 116 diazotrophic Paenibacillus strains
  • The results indicated that that nifEN is not necessary for the biosynthesis and the reconstituted anf system composed of 8 genes Fig. 5 Schematic map and nitrogenase activity of the ΔnifBHDK and ΔnifBHDKEN mutants of P. polymyxa and the complementary strains carrying nifB1anfHDGK, nifB2anfBHDGK, nifB2E2N2anfBHDGK of P. forsythia T98, respectively and the complementary strains carrying nifB1vnfHDGK, nifB2vnfHDGK, nifB2vnfHDGKEN of P. zanthoxyli JH29, respectively. a Schematic map of the P. polymyxa ΔnifBHDK and P. polymyxa ΔnifBHDKEN mutants and the complementary strains. b The nitrogenase activity of the P. polymyxa ΔnifBHDK and P. polymyxa ΔnifBHDKEN mutants and the complementary strains
  • Our analyses have revealed that all nifB genes in Paenibacillus fall into 4 classes and their encoded products have a N-terminal S-adenosyl methionine-radical domain linked to a C-terminal NifX-like domain
  • We have found that some ­N2-fixing Paenibacillus strains possess NifX-like protein that shows higher sequence similarity value with the C-terminal domain of NifB compared with that of NifX protein family
  • The reconstituted anf system comprising 8 genes and vnf system comprising 10 genes support synthesis of Fe-nitrogenase and V-nitrogenase in Paenibacillus background, respectively

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