Vibrio cholerae ghosts (VCG) exert immunomodulatory effect on dendritic cells for enhanced antigen presentation and induction of protective immunity

We previously showed that the Vibrio cholerae ghost platform is an effective delivery system for vaccine antigens promoting the induction of substantial immunity in the absence of external adjuvants

Francis O Eko; Jayanti Mania-Pramanik; Roshan Pais; Qing Pan; Daniel M N Okenu; Arieian Johnson; Chris Ibegbu; Cheng He; Qing He; Raedeen Russell; Carolyn M Black; Joseph U Igietseme

2014

Scholarcy highlights

  • We previously showed that the Vibrio cholerae ghost platform is an effective delivery system for vaccine antigens promoting the induction of substantial immunity in the absence of external adjuvants
  • VCG-pulsed dendritic cell showed increased secretion of proinflammatory cytokines and expression of co-stimulatory molecules associated with DC maturation in response to stimulation with UV-irradiated chlamydial elementary bodies
  • The present study demonstrated that VCGs activate DCs leading to the surface expression of co-stimulatory molecules associated with DC activation and maturation and enhancement of protective immunity induced by a chlamydial antigen
  • The results indicate that the immunoenhancing activity of VCG for increased T-cell activation against antigens is mediated, at least in part, through DC triggering
  • Bone marrow-derived dendritic cells in the absence of chlamydial antigen did not produce appreciable levels of cytokines. These results indicate that chlamydial antigen presentation to immune CD4+ T cells by BMDCs is enhanced by VCG, leading to the production of significantly higher levels of IFN-γ
  • No significant differences in viability of BMDCs incubated with and without VCG were detected
  • The interaction of VCGs with BMDC stimulated the expression of co-stimulatory molecules associated with DC activation and maturation
  • Vibrio cholerae ghost platform could be harnessed as immunomodulators to target antigens to dendritic cell for enhancement of protective immunity against microbial infections

Need more features? Save interactive summary cards to your Scholarcy Library.