A simple way to identify non-viable cells within living plant tissue using confocal microscopy

We show that this dye and its spectral variants, SYTOX blue and SYTOX orange, can be used to identify non-viable cells within plant tissue

Elisabeth Truernit

2008

Scholarcy highlights

  • Plant cell death is a normal process during plant development
  • Plant cell death is an integral part of plant growth and development and can occur as a reaction to wounding or pathogen attack
  • Spectral variants of SYTOX dyes stain the nuclei in fixed plant tissue To assess the property of the different SYTOX dyes and their suitability for the staining of non-viable cells, embryos of Arabidopsis thaliana were dissected from their seed coats, fixed with 4% paraformaldehyde, and stained with SYTOX green, blue, or orange for 5 minutes
  • Due to the scattering of laser light in deeper tissue layers, which is usually observed during live plant imaging 14, the fluorescent signal appeared weaker towards the centre of the plant tissues
  • With the normal constraints of Confocal laser scanning microscopy on live plant tissue we found the fluorescent SYTOX dyes, SYTOX green, blue, and orange, useful and convenient for the identification of non-viable plant cells within living plant tissue
  • The SYTOX dyes will be a useful tool for the study of plant development, physiology, and/or environmental responses

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