Characterization of the stress associated microRNAs in Glycine max by deep sequencing

We investigated the evolutionary conservation features of the identified miRNAs in soybean by comparing them to Arabidopsis thaliana, rice, Zea mays, Medicago truncatula, Sorghum bicolor, Triticum aestivum, Vitis vinifera, brassica and Pinus according to their sequence similarity

Haiyan Li

2011

Scholarcy highlights

  • Plants involved in highly complex and well-coordinated systems have evolved a considerable degree of developmental plasticity, minimizing the damage caused by stress
  • More than 36 million original sequencing tags were produced with approximately 9-10 million raw reads from each library
  • The total numbers of sequence reads in four RNA libraries were similar, the size distribution of sequence tags was substantially different
  • The 24 nt reads constitute 25.90% and 22.14% in drought and mock libraries, while they only account for 15.69% in the alkalinity induced library
  • We investigated the evolutionary conservation features of the identified miRNAs in soybean by comparing them to Arabidopsis thaliana, rice, Zea mays, Medicago truncatula, Sorghum bicolor, Triticum aestivum, Vitis vinifera, brassica and Pinus according to their sequence similarity
  • Little is known about the functions of miRNAs in abiotic stress responses in Glycine max
  • This study has important implications for further identification of gene regulation under abiotic stresses and significantly contributes a complete profile of miRNAs in Glycine max

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