Recombinant human interleukin-11 stimulates megakaryocytopoiesis and increases peripheral platelets in normal and splenectomized mice

We have examined the effects of rhIL- 1 1 on hematologic cell counts and megakaryocytopoiesis in normal and splenectomized mice

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Scholarcy highlights

  • The effects of recombinant human interleukin-11 on in vivo mouse megakaryocytopoeisis were examined
  • INTERLEUKIN- 1 1 was originally identified as a factor produced by IL- I-stimulated PU-34 bone marrow stromal cells that could stimulate proliferation of the IL-6dependent T1165 plasmacytoma.' This biologicalactivity was used to screen a cDNA library generated from IL-l-stimulated PU-34 mRNA expressed in COS cells
  • A number of reports have described multiple effects of rhIL-1 I on megakaryocytopoieisisin vitro.7-" In serum-depleted murine bone marrow cultures, addition of rhIL-I1 increased megakaryocyte size, megakaryocyte ploidy, and acetylcholinesteraseactivity.8It has been shown to synergize with IL-3 to stimulate murine and human megakaryocyte colony formation.7s921S0imilar megakaryocytic stimulatory activities have been described for other cytokines, including IL-6 and leukemia inhibitory factor.8,'2-'6
  • In this report we have examined the effects of in vivo administration of recombinant human interleukin-11 to normal and splenectomized mice
  • Administration of rhIL-11 to normal mice resulted in a marked stimulation of megakaryocytopoiesis and a corresponding increase in peripheral platelet counts
  • The data presented in this report show that treatment of normal or splenectomized mice with rhIL- 11 stimulated megakaryocyte progenitors, endoreplication of bone marrow megakaryocytes, and increased peripheral platelet counts

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