Ultrastructural localization of bcl-2 protein.

The results described are in agreement with a previous description of a peri-nuclear distribution for bcl-2

P Monaghan; D Robertson; T A Amos; M J Dyer; D Y Mason; M F Greaves

2011

Scholarcy highlights

  • The bcl-2 gene was first described in follicular B-cell lymphomas with the t(1418)translocation
  • Labeled preparations of lymphoma cell lines K422 and SU-DHG4 gave similar staining pattems with bcl-2 monoclonal antibodies #100. bcl-2 antigen was present in the perinuclear envelopeas wcll as in regions of the cytoplasm
  • K231 lymphoma cell processed by Progressive Lowering of Temperature and labeled with MAb MOO. bcl-2 is locallzed in three areas: the peripheral region of mitochondria
  • Initial results obtained by electron microscopy gave reproducible results, but relatively low levels of labeling were observed compared with that seen in the same cellsstained by immunocytochemistry for light microscopy
  • The most likely explanation for the discrepancy is lability of the bcl-2 epitopes detected by monoclonal antibodies
  • Samples of SU-DHL-4 cells were initially prepared by the PLT method, which involves a brief aldehyde fixation, followed by low-temperature embedding and polymerization at - 50°C
  • The consistency of the peripheral location of the majority of the gold label, regardless of cell type and processing method, indicates that bcl-2 is associated with the outer region of the mitochondria

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