In vivo formation of H2O2 in red cells during exposure to hyperoxia

The toxic effect of 100% oxygen under high pressure ' on red cells has been recognized for several years

William P. Johnson; Devenia Jefferson; Charles E. Mengel

2008

Scholarcy highlights

  • The toxic effect of 100% oxygen under high pressure' on red cells has been recognized for several years
  • The data are consistent with the hypothesis that H202 is formed or accumulated in excess in red cells of tocopherol-deficient mice, an effect that is enhanced in the presence of hyperoxia
  • Since various prooxidant drugs had been shown to cause H202 to form in RBC's, in vitro and in vivo, the present studies were undertaken to determine if H202 was generated or accumulated in excess in RBC's in vivo during OHP
  • 1 Abbreviations used in this paper: AT, aminotriazole; CA, catalase activity, OHP, 100%o oxygen under high pressure
  • The development and use of oxygen under high pressure in hyperbaric chambers for medical purposes made these effects of considerable practical importance since the in vivo levels of alveolar, venous, and arterial blood oxygen tensions reached during OHP far exceed those which are achieved by any other means
  • Use of aminotriazole for determination of There were no significant differences in the RBC
  • It is of particular note that tocopherol-deficient animals with AT at room air conditions had significantly lower catalase levels, which suggests that tocopheroldeficient animals spontaneously generate or accumulate an excess quantity of H202
  • The fact that immediately after oxygen under high pressure, in tocopherol-deficient mice, those cells which contained the high levels of lipid peroxides contained high levels of H202 would seem to link these two phenomena

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