Muscular Contractile Failure in Septic Patients

We studied NOS2 expression and activity and its role in contractile function in samples from rectus abdominis muscle obtained during surgical procedure in 16 septic patients and in 21 controls

SOPHIE LANONE; ALEXANDRE MEBAZAA; CHRISTOPHE HEYMES; DOMINIQUE HENIN; JUAN JOSE PODEROSO; YVES PANIS; CHRISTINE ZEDDA; TIMOTHY BILLIAR; DIDIER PAYEN; MICHEL AUBIER; JORGE BOCZKOWSKI

2013

Scholarcy highlights

  • Skeletal muscle failure is a frequent manifestation of sepsis that affects prognosis and rehabilitation by impairing respiration and ambulation
  • NOS2 mRNA was detected by semiquantitative reverse transcriptase polymerase chain reaction
  • The primer pair for amplification of our internal standard showed the presence of a 452 bp in all of the mRNA samples tested. the PCR reaction products using human NOS2-specific primers showed a clear band at the predicted size of 388 bp in muscles from septic patients, whereas a very slight band was observed in samples from controls
  • This study shows that NOS2 mRNA, protein, and activity were significantly higher in the rectus abdominis muscle of septic as compared to control patients
  • The slight signal from NOS2 mRNA and protein detected in samples from control patients was reproducibly observed
  • Despite improved therapy and better understanding of the mechanisms underlying the pathogenesis of sepsis, mortality rate remains greater than 40%
  • As recently reported, a slight constitutive NOS2 expression not related to the presence of inflammatory processes may occur in human skeletal muscle
  • This concentration of peroxynitrite is in the range of the one generated in the muscles of septic patients during 12 h, this time corresponding to the average estimated period of sepsis before the rectus abdominis was sampled

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