Hypoxia Regulates Vascular Endothelial Growth Factor Gene Expression in Endothelial Cells

We report in the present study that pulmonary artery endothelial cells do not express Vascular endothelial growth factor under basal conditions; significant VEGF mRNA levels accumulate when these cells are exposed to hypoxia

Yuxiang Liu

2012

Scholarcy highlights

  • Vascular endothelial growth factor is a potent mitogen specific for endothelial cells
  • We report in the present study that pulmonary artery endothelial cells do not express VEGF under basal conditions; significant VEGF mRNA levels accumulate when these cells are exposed to hypoxia
  • Northern blot analysis of total RNA showed a single 3.7-kb VEGF message in RNA isolated from hypoxic endothelial cells, but no signal was detected in normoxic cell RNA. β-Actin mRNA levels were not altered by hypoxia under these conditions
  • VEGF mRNA levels increased by 6 hours of hypoxia, when the Po2 is ≈60 mm Hg, and remained elevated at 48 hours
  • We report in the present study that pulmonary artery endothelial cells are capable of expressing VEGF under conditions of hypoxia by increasing the transcriptional rate of the gene
  • The measured Po2 in the media was 18 to 20 mm Hg under 0% O2 conditions and 130 mm Hg under 21% O2 conditions, as we previously reported
  • We have identified a hypoxia-responsive enhancer in the promoter region of the human VEGF gene, which includes a 28-bp element that is sufficient to mediate the upregulation of VEGF gene transcription
  • The relative contributions of each element may vary according to cell type, such that the 28-bp enhancer identified in the present study may be important in the hypoxic response of endothelial cells, whereas the regions reported by Minchenko et al may play a role in regulating Vascular endothelial growth factor expression in HeLa cells

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