Circulating Tumor Cell Analysis in Patients with Progressive Castration-Resistant Prostate Cancer

We show that circulating tumor cells isolated and enumerated from patients with progressive prostate cancer on androgen depletion represent true neoplastic cells that can be profiled at the protein level and for chromosomal changes by fluorescence in situ hybridization

David R. Shaffer

2007

Scholarcy highlights

  • To better direct targeted therapies to the patients with tumors that express the target, there is an urgent need for blood-based assays that provide expression information on a consistent basis in real time with minimal patient discomfort.We aimed to use immunomagnetic-capture technology to isolate and analyze circulating tumor cells from small volumes of peripheral blood of patients with advanced prostate cancer
  • We show that CTCs isolated and enumerated from patients with progressive prostate cancer on androgen depletion represent true neoplastic cells that can be profiled at the protein level and for chromosomal changes by fluorescence in situ hybridization
  • The present study showed that cells isolated from patients with progressive castration-resistant disease with an automated CTC capture technology had molecular features of malignant prostate epithelial cells
  • We showed that two independent laboratories obtained similar cell counts for the same patient samples, and that the results were consistent whether the samples were processed within 24 or up to 72 and 96 h after phlebotomy
  • CTCs can be enumerated in all patients, even when cell counts are too low to allow molecular characterization by FISH or immunofluorescence
  • Reasoning that the CTC numbers estimated using different techniques should be similar, we used flow cytometry to reanalyze the epithelial cell adhesion molecule antibody-enriched, immunofluorescently stained cells that remained after immunomagnetic selection
  • Reasoning that the circulating tumor cells numbers estimated using different techniques should be similar, we used flow cytometry to reanalyze the epithelial cell adhesion molecule antibody-enriched, immunofluorescently stained cells that remained after immunomagnetic selection

Need more features? Save interactive summary cards to your Scholarcy Library.