Approaches and Methods to Measure Oxidative Stress in Clinical Samples: Research Applications in the Cancer Field

We provide an overview of recent findings of oxidative stress in patients with different types of cancer

Meghri Katerji; Maria Filippova; Penelope Duerksen-Hughes

2019

Scholarcy highlights

  • Reactive oxygen species are chemically reactive molecules containing oxygen that play several beneficial roles for the organism
  • A detailed understanding regarding the link between oxidative stress and pathogenesis can be exploited to assess disease status as well as to develop preventive and therapeutic strategies in humans
  • Direct ROS determination is a valuable and promising oxidative stress biomarker that can reflect on disease status
  • “footprints” of oxidative stress are extremely stable and provide a more reliable approach to evaluate oxidative stress in clinical samples. While some of these modifications only reflect the local degree of oxidative stress, others have a direct effect on the function of target molecules
  • Sample processing should be performed with caution to ensure their stability and to avoid the possibility that biomolecules may become oxidatively damaged during their isolation
  • Some papers report low antioxidant status in cancer samples, explaining it as a loss of their protective capacity due to high oxidative stress, while others interpret the findings of high levels/activities of the antioxidants as an adaptive response mechanism to detoxify oxidative stress-related harmful metabolites
  • It is advisable to determine the total antioxidant status by evaluating all antioxidants simultaneously without excluding their interactions with each other

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