Role of intracellular calcium in hydrogen peroxide-induced renal tubular cell injury

In the present study we examined the role of intracellular free Ca2+ in H2O2-mediated injury to LLC-PK1 cells, a renal tubular epithelial cell line

N. Ueda; S. V. Shah

2017

Scholarcy highlights

  • Both reactive oxygen metabolites and calcium have been implicated in ischemic and toxic renal tubular cell injury
  • In the present study we examined the role of intracellular free Ca2+ in H2O2-mediated injury to LLC-PK1 cells, a renal tubular epithelial cell line
  • At 120 min after exposure of cells to 5 mM H2O2, irreversible cell injury was reduced from 45 +/- 8 to 9 +/- 1% by quin-2
  • The acetoxymethyl ester of quin-2 and BAPTA/AM did not interfere with the trypan blue exclusion assay or scavenge H2O2
  • Preventing mobilization of Ca2+ from intracellular storage sites using 8-(N,N-dimethylamino)octyl 3,4,5-trimethoxybenzoate hydrochloride significantly reduced the rise ini and prevented H2O2-mediated cytotoxicity to LLC-PK1 cells.(ABSTRACT TRUNCATED AT 250 WORDS)

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