A myogenic regulatory gene, qmf1, is expressed by adult myonuclei after injury

We examined the role of Myogenic regulatory factors in the repair of adult muscle using a model of stretch-induced injury in 5-wk-old chickens

Z. A. Eppley

2017

Scholarcy highlights

  • Myogenic regulatory factors induce differentiation in developing muscle
  • We examined the role of MRFs in the repair of adult muscle using a model of stretch-induced injury in 5-wk-old chickens
  • The anterior latissimus dorsi muscle was stretched by loading the wing with 10% of body weight, while the contralateral muscle served as a control
  • At various intervals, chickens were killed by CO2 asphyxiation and the muscles were frozen
  • Slot hybridizations showed that the onset of high qmf expression occurred as early as 0.5 h, which was before regenerative processes involving satellite cell proliferation were observed
  • In situ hybridization showed that maximal qmf expression varied spatially with > 60% of the nuclei within active fascicles being positive
  • We conclude that myogenic regulatory factors are not permanently repressed after embryonic development and that derepression plays a role in the repair of terminally differentiated myofibers

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