Viral DNA and mRNA expression correlate with the stage of human immunodeficiency virus (HIV) type 1 infection in humans: evidence for viral replication in all stages of HIV disease

We have studied viral DNA, genomic RNA, and spliced mRNA expression of human immunodeficiency virus type 1 in infected patients with a quantitative polymerase chain reaction assay

N L Michael; M Vahey; D S Burke; R R Redfield

2020

Scholarcy highlights

  • The results presented here indicate that human immunodeficiency virus type 1 expression at the level of viral DNA and RNA increases with the decline in CD4+ T-cell count and the progression of disease in vivo
  • Precedent exists for a correlation between increasing viral burden and disease progression in HIV-1 disease from both semiquantitative viral culture data and polymerase chain reaction studies from infected peripheral blood mononuclear cells
  • We extend these findings to the actively expressing component of viral burden by demonstrating an increasing load of viral RNA in the PBMCs of infected patients as the disease progresses
  • Cocultivation is further removed from a direct measurement of viral load in that viral recovery is assayed by indirect measurements such as supernatant reverse transcriptase activity and p24 antigen detection
  • A previous report of viral RNA detection using PCR in PBMCs from infected patients showed a correlation between HIV-1 genomic RNA and disease progression, but this study was both nonquantitative and included many patients on active antiretroviral therapy
  • We have shown that the actively expressing component of viral burden increases with disease progression in human immunodeficiency virus type 1 infection

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